The First Presbyterian Church at Main and Roxboro streets is the congregation's third at that location. Presbyterians organized in Durham in 1871 and built a wood-frame place of worship four years later. A new, Gothic Revival building with a 70-foot spire went up in 1890. It was replaced by the present building in 1916, which did not change the Gothic Revival design.

Its sanctuary is distinguished by tracery stained-glass windows by the German Von Grechton glassworks.